J. Thomas Beatty
Ph.D. Indiana University, Microbiology (1980); Postdoctoral, Stanford University School of Medicine (1983)
Email: jbeatty [at]interchange.ubc.ca
Office Telephone: 604-822-6896
2350 Health Sciences Mall
Life Sciences Centre
My laboratory's research activities are centered on the general areas of bacterial molecular biology, physiology and genetics, with an emphasis on the regulation of gene expression, and protein structure and function. Some of the techniques used are molecular cloning, gene fusions, site-directed mutagenesis, RNA analyses, and purification of proteins.
One area of research in my laboratory is on gene structure and expression in photosynthetic bacteria. We study changes in transcription initiation, mRNA processing and protein-protein interactions in response to environmental factors such as oxygen concentration and light intensity, and a newly discovered quorum sensing pathway.
The genomes of several species of purple photosynthetic bacteria are being sequenced, with complete or nearly complete sequences available at this time. We are using these and other DNA sequences to discover new genes, to investigate their regulation of expression, and the function of their protein products.
The crystal structures of several purple bacterial photosynthetic reaction centers and light-harvesting complexes have been solved and shown to be highly conserved. We are using this structural information to design and interpret site-directed mutagenesis experiments to address questions about how individual proteins bind to each other and to cofactors, and pathways of proton transfer within and between protein complexes.
We are also studying a novel bacteriophage-like genetic exchange element called the "gene transfer agent", or GTA. The production of GTA is controlled by changes in environmental conditions that are sensed by a cellular signal transduction system, which produces a burst of GTA production in the stationary phase of laboratory cultures. The nature of the signal(s), and the identities and activities of regulatory factors are of great interest to us.
In collaboration with John Madden's group in the Department of Electrical and Computer Engineering (http://www.ece.ubc.ca/~jmadden/), we are investigating the feasibility of using photosynthetic reaction centers in biophotovoltaic devices, in the hopes of creating inexpensive solar cells whose production consumes carbon dioxide. These devices may be able to harvest and store energy in one simple device.
Finally, we are investigating the diversity and ecological activities of photosynthetic bacteria in aquatic environments ranging from stratified lakes to deep ocean hydrothermal vents.