JEMI Volume 18

JEMI
Volume 18
Fall 2013 / Winter 2014

Physiology and Metabolism

Sub-Inhibitory Concentrations of Kanamycin May Induce Expression of the Aminoglycoside Efflux Pump acrD Through the Two-Component Systems CpxAR and BaeSR in Escherichia coli K-12
Scott Besse, Daniel Raff, Michael Thejomayen, Paxton Ting
J. Exp. Microbiol. Immunol. Vol. 18: 1 – 6

AcrS is an Activator of acrD Expression in Escherichia coli K-12 Following Exposure to Sub-inhibitory Concentration of Kanamycin Pretreatment
Mariam Emami, Stella Xu, Thomas Chan
J. Exp. Microbiol. Immunol. Vol. 18: 7 – 11

Expression of rprA, a Regulator of RpoS Translation, Increases Tolerance to Kanamycin in RcsB-deficient Escherichia coli K-12.
Amanda Amirault, Connie Kuo, Brent Sage
J. Exp. Microbiol. Immunol. Vol. 18: 12 – 17

Heat Induced RpoS Expression May Inhibit RpoD-dependent Expression of β-galactosidase in Escherichia coli B23
Arif Arif, Maninder Dhaliwal, Baljeet Panasar, Steven Yang
J. Exp. Microbiol. Immunol. Vol. 18: 18 – 23

RpoS May Not Play a Central Role in Coordinating Heat Shock Stress Response and Antibiotic Tolerance
Raizell Castro, Meghan Dougan, Laurence Rioux, Becky Zhang
J. Exp. Microbiol. Immunol. Vol. 18: 24 – 29

Cross-Protection Against Tetracycline Conferred by Thermal Stress in Escherichia coli May be Independent of rpoS and otsA
Shivani Gounder, Yulia Merkulova, Kelsie Nixon, Tina Wang
J. Exp. Microbiol. Immunol. Vol. 18: 30 – 35

RpoS is not Necessary to Increase Heat Tolerance in Escherichia coli BW25113 Following Sub-Inhibitory Kanamycin Pre-Treatment
Mandy Feng, Jordan Henriksen, Carly Wong, Ryan Yen
J. Exp. Microbiol. Immunol. Vol. 18: 36 - 40

The Molecular Chaperone DnaJ is Involved in the Peroxide-Induced Oxidative Stress Response in Escherichia coli.
Kang Yang, Linda Yang, and James Liu
J. Exp. Microbiol. Immunol. Vol. 18: 41 – 47

Adaptive Kanamycin Resistance Induced by the Stringent Response in Escherichia coli is Not Dependent on katE and katG
Jenny Chen, Bonny Chow, Patrick Hopkins, Joseph Tio
J. Exp. Microbiol. Immunol. Vol. 18: 48 – 53

Differential Effects of Sucrose or NaCl Osmotic Shock on β-galactosidase Activity in Escherichia coli BW25993 is Dependent on Normalization Method by Total Viable Count or Total Protein Content
Judy Chang, Shannon Coleman, Young Eun Lee, Tegan Schellenberg-Beaver
J. Exp. Microbiol. Immunol. Vol. 18: 54 – 59

Growing Escherichia coli at a Range of Temperatures to Change Expression of OmpA Did Not Affect the Efficiency of Transformation
Martin (Chien Hao) Chen, Jacky Li, Jonathan Ng, Joshua (Ting Hua) Wu
J. Exp. Microbiol. Immunol. Vol. 18: 60 – 64

EnvZ is Not Essential for the Upregulation of OmpC Following Treatment with Sublethal Kanamycin in Escherichia coli
Jasmine Lo, Tessa Van Tol, Stephanie Yeung, Kevin Zou
J. Exp. Microbiol. Immunol. Vol. 18: 65 – 69

The Escherichia coli K12 Capsule Does Not Confer Resistance to Either Tetracycline or Streptomycin
Sunjit Parmar, Alykhan Rajwani, Sarpreet Sekhon, Kartik Suri
J. Exp. Microbiol. Immunol. Vol. 18: 76 – 81

Phthalate Confers Protection Against UV-A Irradiation but is an Uncompetitive Inhibitor of Bovine Catalase at High Concentrations
David Choy, David Lee, Jeffrey Ma, Kelvin Wong
J. Exp. Microbiol. Immunol. Vol. 18: 82 - 86

Genetics and Molecular Biology

The Temperature of the Cold Shock during Temperature Shock Transformation Has No Effect on Transformation Efficiency of Escherichia coli
Adrian Chu, Cindy Liu, Fabian Tam, Jessica Zhang
J. Exp. Microbiol. Immunol. Vol. 18: 87 - 90

Nucleotide Composition Immediately Flanking the DNA Overhangs Generated by Restriction Endonucleases Was Not Shown to Contribute to the Ligation Efficiency of T4 DNA Ligase
ChiaYing Chen, SeungHyun Choi, Yue Hu, Mei Mu Zi Zheng
J. Exp. Microbiol. Immunol. Vol. 18: 91 - 95

Attempts to Construct an Enlarged pUC19 via Insertion of HindIII-digested Coliphage λ DNA
Muhamed Amirie, Isabelle Cheng, Joanne Cho, Viet Vu
J. Exp. Microbiol. Immunol. Vol. 18: 96 - 101

Construction of pANPT, a Novel pBR322-derived Plasmid with Deletion of the Tetracycline Resistance Gene
Al-Hassan Al-Shaibani, Dashaylan Naidoo, Kathy Pan, Michelle Tran
J. Exp. Microbiol. Immunol. Vol. 18: 102 - 107

Introduction of a Specific Point Mutation into the RNA II Locus on a rop-deficient pBR322 Plasmid
Kevin Kuchinski, Harveen Grewal
J. Exp. Microbiol. Immunol. Vol. 18: 108 – 113

Improved Sensitivity of the in vivo lux Reporter System in Measuring Reactive Oxygen Species during Antibiotic Treatment of Escherichia coli
Carl Chua, Sharon Lau, Sadiq Vali, and Yin Shu Yang
J. Exp. Microbiol. Immunol. Vol. 18: 114 - 120

Attempted Construction of a soxR::PsoxS::lacZ Recombinant pBR322 Plasmid as a Sensitive Reporter of Internal Reactive Oxygen Species in Escherichia coli DH5α.
Dominique Haag, Tony Hui, Seo Young Kim, Jane Nguyen
J. Exp. Microbiol. Immunol. Vol. 18: 121 - 128

Construction of a pBAD24 Vector Containing σ32: Restoration of Function in σ32 Knockout Escherichia coli
Darson Du, Katherine Lee, Samantha Mori, Michael Wu
J. Exp. Microbiol. Immunol. Vol. 18: 129 - 133

Attempted construction of an arabinose-inducible pBAD28 recombinant expression vector containing acrD, an Escherichia coli aminoglycoside efflux porin
Mahmud Iqbal, Ke Li, David Reading, Kia Shahtaheri
J. Exp. Microbiol. Immunol. Vol. 18: 134 - 138

Partial Confirmation of Single katG and katE Knockouts and Double katG/katE Knockouts Created from Isogenic Background of Escherichia coli K-12 Strains
Desmond Hui, Shahrzad Khaiat, Tanya Uy, Haocheng Xu
J. Exp. Microbiol. Immunol. Vol. 18: 139 - 145

Banding Profile of Rep-PCR Experiments Differs With Varying Extension Times and Annealing Temperatures
Daniel Korvin, Colin Graydon, Larisa McNeil, Marta Mroczek
J. Exp. Microbiol. Immunol. Vol. 18: 146 - 149

Phage Biology

T7 Production in Escherichia coli B23 Is Not Inhibited By ΦX-174 at a Multiplicity of Infection of 10
Cordelia Cheng, Natasha Lee, Armin Mortazavi, and Caitlin Pritchard
J. Exp. Microbiol. Immunol. Vol. 18: 150 – 155

Co-inoculation of Escherichia coli B23 by T4 and T7 Bacteriophages Results in Competition Shown by an Overall Drop in Phage Progeny
Khue-Tu Nguyen, Karen Simmons, Igor Tatarnikov
J. Exp. Microbiol. Immunol. Vol. 18: 156 – 161

T4 Bacteriophage May Not Inhibit Transcription of T7 Bacteriophage Genes rpol and gp10a During Co-infection of Escherichia coli
Anthony Heggen, Angela McLaughlin, Gordon Russell, Angela Zhang
J. Exp. Microbiol. Immunol. Vol. 18: 162 - 166