JEMI Volume 13

JEMI
Volume 13
August 2009

Physiology and Metabolism

The Effect of Ionic (NaCl) and Non-ionic (Sucrose) Osmotic Stress on the Expression of β-galactosidase in Wild Type E.coli BW25993 and in the Isogenic BW25993ΔlacI Mutant
Christine Cheung, Jenny Lee, Jiyun Lee and Olena Shevchuk
J. Exp. Microbiol. Immunol. Vol. 13: 1 – 6

Effect of Sequential Treatment with High Temperature and Hyperosmolarity or Low pH on RpoS Expression and Cell Viability in Escherichia coli B23
Yun-Wen Hsu, Lucia Lam, Joohyun Lee and Ashish Sharma
J. Exp. Microbiol. Immunol. Vol. 13: 7 – 12

The Effect of Osmotic Shock on RpoS Expression and Antibiotic Resistance in Escherichia coli
Ying-Min Huang, Bernard Kan, Yue Lu and Sharon Szeto
J. Exp. Microbiol. Immunol. Vol. 13: 13 – 17

RpoS-Dependant Mechanism is Required for Cross Protection Conferred to Hyperosmolarity by Heat Shock
Parmida Behmardi, Emendeep Grewal, Yoojun Kim and Han Na Yang
J. Exp. Microbiol. Immunol. Vol. 13: 18 – 21

Effects of Reduced Capsular Polysaccharide on Kanamycin Resistance in Escherichia coli B23 Cells
Joanna Kam, Xi L. Luo and Hee-yun A. Song
J. Exp. Microbiol. Immunol. Vol. 13: 22 – 28

Effect of Growth at Sub-lethal Concentrations of Kanamycin on the Cell Membrane Integrity and Amount of Capsular Glucuronic Acid in Wild-type Escherichia coli and Strain with a cpsB Mutation
Sehyun Cho, Joyce Law and Chi Kwan Ng
J. Exp. Microbiol. Immunol. Vol. 13: 29 – 35

The Role of wza in Extracellular Capsular Polysaccharide Levels During Exposure to Sublethal Doses of Streptomycin
Iman Naimi, Marjan Nazer, Louie Ong and Emily Thong
J. Exp. Microbiol. Immunol. Vol. 13: 36 – 40

The Effect of Aberrant Protein Synthesis on the Production of Capsular Polysaccharide in Escherichia coli B23
Andrew L. Wong, Daniel Y. Fong, Guilaine K. Boyce and Minhee R. Kim
J. Exp. Microbiol. Immunol. Vol. 13: 41 – 46

The Effect of Sub-Inhibitory Streptomycin on Capsular Polysaccharide Production and Streptomycin Resistance in Escherichia coli
David Fowler, Jeffrey Hu, Philip Hou and Carolyn Wong
J. Exp. Microbiol. Immunol. Vol. 13: 47 – 52

Sublethal Kanamycin Induced Cross Resistance to Functionally and Structurally Unrelated Antibiotics
Lawrence Xinan Chen, Sarah He, Chen Li and Jennifer Ryu
J. Exp. Microbiol. Immunol. Vol. 13: 53 – 57

The Role of Alginate in P. aeruginosa PAO1 Biofilm Structural Resistance to Gentamicin and Ciprofloxacin
Laura A. Cotton, Rebecca J. Graham and Rose J. Lee
J. Exp. Microbiol. Immunol. Vol. 13: 58 – 62

katE Complementation Fails to Protect Against UV-A-Mediated Killing in Catalase-Deficient Escherichia coli
Mona Cheng, Anna Chow, Julie Ho and Beryl Luk
J. Exp. Microbiol. Immunol. Vol. 13: 63 – 66

Effects of the PitA Phosphate Uptake System and Calcium Ion Channel on Transformation Efficiency in Escherichia coli K-12 Cells
Amy Prangnell, Kali Romano, Casey Wong and Gary Yip
J. Exp. Microbiol. Immunol. Vol. 13: 67 – 70

Effects of Temperature-Induced Changes in Membrane Composition on Transformation Efficiency in E. coli DH5α
Rebecca Brooke, Jessica Hopkins, Emily Laflamme and Paige Wark
J. Exp. Microbiol. Immunol. Vol. 13: 71 – 74

Comparison Between Transformation Efficiencies in Rod-shaped and Spherical-shaped Escherichia coli Strains
Mona Habibi, Pamela Liem, Wendy Luong and Ekaterina Netchaeva
J. Exp. Microbiol. Immunol. Vol. 13: 75 – 78

Analysis and Comparison of β-galactosidase Production in Escherichia coli BW25993/ΔlacI and MG1655/ΔlacI Mutants During Conditions of Growth and Catabolite Repression
Bao R. Huo, Roger Jen, Petra Lolić and Derek To
J. Exp. Microbiol. Immunol. Vol. 13: 79 – 82

Evaluation of Stringency Elicited by Antibiotics on Plasmid Copy Number of pBR322 Per Genome in DH5α Escherichia coli Cells
May Au, Jonathan Ip, Iris Tao and Monan Angela Zhang
J. Exp. Microbiol. Immunol. Vol. 13: 83 – 88

Phage Biology

The Reduction of T7 Phage Adsorption in Escherichia coli B23 Cells Treated with Sub-Lethal Levels of Kanamycin
Joanne Bleackley, Jesse Cooper, Monica Kaminski and Stephanie Sandilands
J. Exp. Microbiol. Immunol. Vol. 13: 89 – 92

Genetics and Molecular Biology

Consistency and Applicability of the λ-Red Recombinase System in Escherichia coli Tested on the Target Genes lacI and phoA
Petra Schreiner
J. Exp. Microbiol. Immunol. Vol. 13: 93 – 99

Investigating Whether NdeI-Cut Ends are Available to Incorporate Deoxynucleotides
Lydia Liao
J. Exp. Microbiol. Immunol. Vol. 13: 100 – 103

Optimization and the Robustness of BOX A1R PCR for DNA Fingerprinting Using Trout Lake E. coli Isolates
Alfred Lee and Eva Wong
J. Exp. Microbiol. Immunol. Vol. 13: 104 – 113

Subcloning emoA into pET32a(+), pET32a(+)DtrxA/+fre and pET32a(+)DtrxA to Assess the Relative Effect of Thioredoxin A and Flavin Oxidoreductase on emoA Solubility
Hok Khim Fam
J. Exp. Microbiol. Immunol. Vol. 13: 114 – 118

Attempted Construction of a pET32a(+) Vector Containing EDTA Monooxygenase A
Andrew Cameron, Stuart Gray, Anastasia Sribnaia and Tracy Withrow
J. Exp. Microbiol. Immunol. Vol. 13: 119 – 124