JEMI Volume 16

JEMI
Volume 16
Fall 2011 / Winter 2012

Physiology and Metabolism

Effects of Capsule Induction and Stripping on the Resistance of Escherichia coli B23 to Tetracycline
Abdimalik Khalif and Dwayne da Silva
J. Exp. Microbiol. Immunol. Vol. 16: 1 – 4

Osmotic Stress Induces Kanamycin Resistance in Escherichia coli B23 through Increased Capsule Formation
Anita Kuzhiyil, Yoojin Lee, Amy Shim, and Anqi Xiong
J. Exp. Microbiol. Immunol. Vol. 16: 5 – 10

Treatment with Sub-inhibitory Kanamycin Induces Adaptive Resistance to Aminoglycoside Antibiotics via the AcrD Multidrug Efflux Pump in Escherichia coli K-12
Kiran Sidhu, Martha Talbot, Kirstin Van Mil, and Meghan Verstraete
J. Exp. Microbiol. Immunol. Vol. 16: 11 – 16

Increased Intracellular Hydrogen Peroxide Observed in Escherichia coli in the Presence of Two Classes of Antibiotics
Andrea Brown, Dara Lo, Chloe Sharpe, and Cindy Tong
J. Exp. Microbiol. Immunol. Vol. 16: 17 – 23

RpoS-Dependent Mechanisms are Needed for Tetracycline Resistance but Are Not Involved in the Cross-Protection against Other Antibiotics in Escherichia coli
Chiho Ahn, Chiyoon Ahn, Isaac Kim, Junhwan Yoo
J. Exp. Microbiol. Immunol. Vol. 16: 24 – 29

Acid Induced rpoS Expression in Escherichia coli Does Not Induce Cross-Protection against Ceftriaxone
Amy Davis, Jennie Ding, Joey Lo and Zhen Lu
J. Exp. Microbiol. Immunol. Vol. 16: 30 – 35

Pre-treatment with Sub-Inhibitory Kanamycin Has no Effect on RpoS Expression but Increases Heat Tolerance of Escherichia coli BW25113
Nelly Amenyogbe, Jessica Leung, and Yulia Loktionova
J. Exp. Microbiol. Immunol. Vol. 16: 36 – 41

The Role of Alginate and Extracellular DNA in Biofilm-Mediated Pseudomonas aeruginosa Gentamicin Resistance
Milan Aspe, Lionel Jensen, Josh Melegrito, and Michael Sun
J. Exp. Microbiol. Immunol. Vol. 16: 42 – 48

Resistance to Tobramycin in Pseudomonas aeruginosa PA14 and PA14 ΔpelB Co-culture Biofilm through Antibiotic Sequestering
Soujun Chen, Eric Chak-Ting Kong, Jina Lee, and Sean Zhu
J. Exp. Microbiol. Immunol. Vol. 16: 49 – 53

Growth of Bacillus subtilis in Phosphate Limited Media Reduces Susceptibility to Antibacterial Activity of Chitosan
Albert Chiang, C. Michael Honey, Samuel Lau, and Dylan Olver
J. Exp. Microbiol. Immunol. Vol. 16: 54 – 58

Mildly Inhibitory Effects of Sodium Polyanethol Sulfonate and pH on the Growth of Wildtype, lrgAB, and cidAB Mutants of Streptococcus mutans
Jonathan Cheng, Hui Hao Hsueh, Stella Setiawan, and Jeff Wong
J. Exp. Microbiol. Immunol. Vol. 16: 59 – 65

Ligninase Activity Is Not Consistently Predicted by the Presence of Manganese Coordinating Residues in Dyp-like Proteins
Cameron Strachan, Dave VanInsberghe, and Dominique Williams
J. Exp. Microbiol. Immunol. Vol. 16: 66 – 72

Genetics and Molecular Biology

Growth Inhibitory Levels of Salicyclic Acid Decrease Pseudomonas aeruginosa fliC Flagellin Gene Expression
Yifei Dong, Clive Huang, Jacob Park, and Guessy Wang
J. Exp. Microbiol. Immunol. Vol. 16: 73 – 78

Production of a Recombinant Vector to Enable the Study of Thioredoxin Function as a Bound or Detached Solubilizer of Proteinase Inhibitor 2 in a Bacterial Protein Overexpression System
Chris Duronio
J. Exp. Microbiol. Immunol. Vol. 16: 79 – 84

PCR Optimization of BOX-AIR PCR for Microbial Source Tracking of Escherichia coli in Waterways
Abby Yang and Charles Yen
J. Exp. Microbiol. Immunol. Vol. 16: 85 – 89

Construction of Escherichia coli K-12 Strain Deficient in relA and spot Using the γ Red Site-specific Recombinase System
So Ra Lee
J. Exp. Microbiol. Immunol. Vol. 16: 90 – 95

Construction of the Escherichia coli DL11w-3 Strain with spoT relA Double Deletions
David C.K. Liou
J. Exp. Microbiol. Immunol. Vol. 16: 96 – 100

The Down-Regulation of E. coli OmpF in Response to Sub-Inhibitory Concentrations of Kanamycin Is Not Mediated by MarA
Yang Fei, Vivian Ma, Nina Maerkl, and Paul You
J. Exp. Microbiol. Immunol. Vol. 16: 101 – 107

Rescuing the ompA Deletion Mutant Escherichia coli JW0940 by Reintroducing ompA in the TOPO Cloning Vector pBAD
Aaron Lal, Javier Vela, Justin Yee and Eric Yuan
J. Exp. Microbiol. Immunol. Vol. 16: 108 – 112

Genetic Compensation of E. coli C156 with pBAD-ompA for the Investigation of Conjugation Efficiency
Anthony Hsieh and Esten William
J. Exp. Microbiol. Immunol. Vol. 16: 113 – 118

Deleting the hns Gene for the H-NS Protein in Escherichia coli Reduces the Transformation Efficiency Following the Heath Shock Transformation Protocol
Marc Soloman
J. Exp. Microbiol. Immunol. Vol. 16: 119 – 122

Construction of Catalase Double Knockout Escherichia coli Strain for Isogenic Strain Comparison Studies for the Role of Catalase
Lisa Narita and Sophia Peng
J. Exp. Microbiol. Immunol. Vol. 16: 123 – 128

Construction of pCAWK, a Novel pBR322-derived Plasmid with Insertional Inactivation of the rop Gene
Adriana Airo, Farnaz Changizi, Moochan Kim, and Jennifer Wibowo
J. Exp. Microbiol. Immunol. Vol. 16: 129 – 135

Fatty Acid fabF Mutants of Escherichia coli Show Limitation on Transformation Frequency
Ada Mew and Rhena Yoo
J. Exp. Microbiol. Immunol. Vol. 16: 136 – 138

Phage Biology

Co-Infection of C3000 Esherichia coli with Bacteriophages MS2 and T7 or pX-174 Results in Differential Cell Lysis Patterns
Lara Brewster, Mary Langley, and David Twa
J. Exp. Microbiol. Immunol. Vol. 16: 139 – 143

Methods

An Ultraviolet Sterilization Protocol for Microtitre Plates
Aishwariya Sharma
J. Exp. Microbiol. Immunol. Vol. 16: 144 – 147