JEMI Volume 8

JEMI
Volume 8
December 2005

Genetics and Molecular Biology

Evaluating the Role of G,C-nucleotides and Length of Overhangs in T4 DNA Ligase Efficiency.
  GURNEET BOLA
J. Exp. Microbiol. Immunol. 2005. 8:1-7.

Cloning of λ DNA fragments into pUC19 vector to study the ligation efficiency of NdeI-digested pUC19 and HindIII-digested pUC19 by T4 DNA ligase.
  POLLY CHOW
J. Exp. Microbiol. Immunol. 2005. 8:8-13.

Investigation of the Relationship between Terminal Sequence Variation and the Ligation Efficiency of T4 DNA Ligase on Blunt-ended DNA.
  SUMAN DHESI
J. Exp. Microbiol. Immunol. 2005. 8:14-20.

Investigation of the Effect of NdeI on DNA Fragments Destined for Ligation.
  JEFFREY GAGNON
J. Exp. Microbiol. Immunol. 2005. 8:21-26.

Construction of a Mutant pBR322 Using Site-Directed Mutagenesis to Investigate the Exclusion Effects of pBR322 During Co-transformation with pUC19.
  IVANA KOMLJENOVIC
J. Exp. Microbiol. Immunol. 2005. 8: 27-32.

Analyzing the efficacy of the λ-red recombinase system by site-directed mutagenesis of phoA and lacI in Escherichia coli.
  WILSON LUK
J. Exp. Microbiol. Immunol. 2005. 8:33-39.

Attempt to Construct a Rop+ pUC19 by Using NdeI, AatII, and AfeI Restriction Endonucleases with Separate Blunt and Staggered-end Ligations.
  IRENE NG
J. Exp. Microbiol. Immunol. 2005. 8:40-46.

Investigation of the Ligation Efficiency of Digested λ Fragments of Different Sizes and Compatibility of Ends.
  ALAN TSANG
J. Exp. Microbiol. Immunol. 2005. 8:47-53.

Generation of Intermediate Plasmids for the Investigation of Sequence- Dependent Ligase Activity due to Location of Sequences Relative to the Restriction Endonuclease Nick Site.
  JACKY WONG
J. Exp. Microbiol. Immunol. 2005. 8:54-59.

Cloning of Flavin Reductase into pET32a(+) Expression Vector Lacking the Thioredoxin A Tag to Study Solubility of EDTA Monooxygenase A in Overexpression Systems.
  SIAW HUI WONG
J. Exp. Microbiol. Immunol. 2005. 8:59-66.