STRUCTURAL BIOLOGY SYMPOSIUM
Speaker: Dr. Alba Guarné (Professor, McGill University)
Seminar: Structural basis for targeting and immunity of the Tn7 transposon
Abstract: Tn7 and CRISPR-associated Tn7 transposons (CAST) elements are bacterial transposons unique in their ability to recognize highly specific target sites. The precise Tn7 targeting mechanisms rely on the coordinated actions of multiple Tn7-encoded proteins, including the heteromeric transposase, the AAA+ ATPase adaptor, and the target selection proteins. I will discuss our recent cryo-EM work revealing how the targeting complex assembles. These findings, combined with our previous work on the TnsC adaptor, provide the mechanistic understanding underlying targeting at the attTn7 site, recruitment of the transposase at a strict spacing from the target site, and transposition immunity.
Speaker: Dr. Kelly Kim (Assistant Professor, Michigan State University)
Seminar: Untangling the Complexity of Membrane Protein Biogenesis
Abstract: Membrane proteins play crucial roles in cellular functions, and their malfunction is often associated with devastating human diseases. Ensuring the accurate synthesis, targeting, and insertion of membrane proteins into their designated cellular membranes are vital processes for maintaining proper membrane function and integrity. We explore various aspects of membrane protein biogenesis, with a specific focus on understanding how cells accurately deliver and insert tail-anchored proteins (TAs) into the endoplasmic reticulum (ER) membrane.
LSC 3 (Life Sciences Institute - 2350 Health Sciences Mall) MBIM itsupport@microbiology.ubc.ca America/Vancouver publicSTRUCTURAL BIOLOGY SYMPOSIUM
Speaker: Dr. Alba Guarné (Professor, McGill University)
Seminar: Structural basis for targeting and immunity of the Tn7 transposon
Abstract: Tn7 and CRISPR-associated Tn7 transposons (CAST) elements are bacterial transposons unique in their ability to recognize highly specific target sites. The precise Tn7 targeting mechanisms rely on the coordinated actions of multiple Tn7-encoded proteins, including the heteromeric transposase, the AAA+ ATPase adaptor, and the target selection proteins. I will discuss our recent cryo-EM work revealing how the targeting complex assembles. These findings, combined with our previous work on the TnsC adaptor, provide the mechanistic understanding underlying targeting at the attTn7 site, recruitment of the transposase at a strict spacing from the target site, and transposition immunity.
Speaker: Dr. Kelly Kim (Assistant Professor, Michigan State University)
Seminar: Untangling the Complexity of Membrane Protein Biogenesis
Abstract: Membrane proteins play crucial roles in cellular functions, and their malfunction is often associated with devastating human diseases. Ensuring the accurate synthesis, targeting, and insertion of membrane proteins into their designated cellular membranes are vital processes for maintaining proper membrane function and integrity. We explore various aspects of membrane protein biogenesis, with a specific focus on understanding how cells accurately deliver and insert tail-anchored proteins (TAs) into the endoplasmic reticulum (ER) membrane.